Vol. 30 (Suppl 1) 2009 Neuro endocrinology letters

Preparation of apo-cytochrome b5 utilizing heme transfer to apo-myoglobin.

OBJECTIVES: Cytochrome b5 (cyt b5), a component of endoplasmic reticulum membrane, plays a role in modulation of activity of several cytochromes P450 (CYP). To elucidate the mechanism of such modulations it is necessary to evaluate not only the effect of native cyt b5, but also that of apo-cyt b5. To prepare apo-cyt b5, heme transfer fr.....