2004; 25(1-2): 83-86
PubMed PMID: 15159689
Animals, Antigens, CD13:metabolism, Blood Glucose:analysis, Cathepsin D:metabolism, Cathepsin L, Cathepsins:metabolism, Cysteine Endopeptidases, Down-Regulation, Female, Insulin:physiology, Kidney:enzymology, Leucyl Aminopeptidase:metabolism, Liver:enzymo.
OBJECTIVES: Changes in the activity of alanine aminopeptidase, leucine aminopeptidase and cathepsins D and L in the liver and kidney of male and female of mice, injected with 0.4 IU/kg b.w. insulin for 4 and 8 days.
METHODS: The homogenates of the liver and kidney were taken for examination. The activity of alanine aminopeptidase, leucine aminopeptidase and cathepsins D and L has been determined according to  method.
RESULTS: The activity of alanine aminopeptidase, leucine aminopeptidase, cathepsins D and L in the liver and kidney of male and female of mice decreased in effect of insulin injections for 4 and 8 days.
CONCLUSION: The changes of enzyme activities showed a stimulating effect of the insulin injection on the labilization of lysosomal membranes. The range of the reaction remained in a relationship with the kind of the organ, the type of enzyme, time over which insulin introduced operates in the organism, and with the sex....