Effect of hydrocortisone on the activity of some lysosomal enzymes in mice.

OBJECTIVES: Changes in the activity of cathepsin D and L, alanine aminopeptidase, leucine aminopeptidase, N-acetyl-beta-glucosaminidase, lysosomal arylesterase and lysosomal lipase in the liver and kidney of unselected and selected mice, subjected to 7.5 mg/kg b.w. of hydrocortisone injection for 4 and 8 days.

METHODS: The homogenates of the liver and kidney were subjected to differentiated centrifuging and determination of studied enzymes.

RESULTS: Injection of hydrocortisone caused an increase in the activity of all investigated lysosomal enzymes in the liver and kidney of mice.

CONCLUSION: The reactions of selected mice were stronger in comparison with unselected ones. The highest increase in the activity investigated enzymes was observed after 8 days of hydrocortisone injection.