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NEUROENDOCRINOLOGY
LETTERS
including
Psychoneuroimmunology, Neuropsychopharmacology,
Reproductive Medicine, Chronobiology
and Human Ethology, ISSN 0172–780X
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NEL
Vol.24 Nos.3/4, Jun-Aug 2003
ORIGINAL ARTICLE
Running
Title:
Hormonal function and proliferative activity of thymic cells
in humans: Immunocytochemical correlations
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2003;
24:263–267
pii: NEL243403A19
PMID: 14523368
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Hormonal
function and proliferative activity of thymic cells in humans:
Immunocytochemical correlations
Igor
M. Kvetnoy,1 Victoria O. Polyakova,2 Alexander
V. Trofimov,2 Vadim V. Yuzhakov,3 Alexander
A. Yarilin,4 Emma S. Kurilets, 3 Ludmila N.
Mikhina, 3 Nina I. Sharova 4
& Maria F. Nikonova 4
1.
Ott Institute of Obstetrics and Gynecology, St. Petersburg,
Russia
2. St. Petersburg Institute of Bioregulation and Gerontology,
St. Petersburg, Russia
3. Medical Radiological Research Center, Obninsk, Russia
4. Institute of Immunology, Moscow, Russia
Submitted:
April 24, 2003
Accepted: May 21, 2003
Key
words:
thymus, immunocytochemistry, hormone’ expression, epithelial
cells, proliferation of thymocytes
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Abstract
OBJECTIVES:
The immunocytochemical study of the localization of hormones
in thymic cells has been performed to clarify possible correlations
of their expression with proliferative activity of thymocytes.
METHODS:
We used commercial antibodies to serotonin, melatonin, somatostatin,
glucagon, gastrin, beta-endorphin and histamine, and ABP or
BSP kits for visualization of reaction. Computer image analysis
was used to find correlations between hormone production and
proliferative activity of thymocytes.
RESULTS: Different subpopulations of thymocytes are able to
produce hormones: precursors of T-lymphocytes (CD4–CD8–)
contain serotonin and melatonin, immature cortical cells (CD4+CD8+)
produce only serotonin, mature medullar cells (CD4+CD8–)
show immunoreactivity to serotonin, melatonin, beta-endorphin
and histamine. The expression of serotonin, somatostatin and
gastrin is localized in thymic epithelial cells, formatting
Gassal’s bodies. Proliferative activity of thymocytes
depends from the expression of serotonin and somatostatin
in thymic cells.
CONCLUSION:
The data received testify the expression of different hormones
in human thymic cells and showing by this fact high endocrine
activity of thymus. The presence of correlations between hormonal
expression and cell proliferative activity could be considered
as the bright illustration of important role of neuroimmunoendocrine
mechanisms in the regulation of local thymic homeostasis.
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__________________________________________________________
Copyright © Neuroendocrinology Letters 2003
Society of Integrated Sciences
All rights reserved. No part may be reproduced, stored in a
retrieval system, or transmitted in any form or by any means,
electronic, mechanical, photocopying, recording, or ortherwise,
without prior written permission from the Editor-in-Chief.
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