|
|
NEUROENDOCRINOLOGY
LETTERS
including
Psychoneuroimmunology, Neuropsychopharmacology,
Reproductive Medicine, Chronobiology
and Human Ethology, ISSN 0172–780X
|
|
|
NEL
Vol.24 Nos.3/4, Jun-Aug 2003
ORIGINAL ARTICLE
Chromacin-like peptide in leeches
|
2003;
24:227–232
pii: NEL243403A13
PMID: 14523362
[Read
pdf 150kb]
Buy
article
Check
Out
|
| |
Chromacin-like
peptide in leeches
Michel
Salzet 1 & George Stefano 2
1.
Laboratoire de Neuroimmunologie des Annélides, Université
des Sciences et Technologies de Lille, Villeneuve d’Ascq
Cedex, France.
2.
Neuroscience Research Institute, State University of New York
College at Old Westbury, Old Westbury, NY 11568-0210, USA.
Submitted:
November 6, 2002
Accepted: December 16, 2002
Key
words:
neuroimmunity, chromacin-like peptide, invertebrate, lophotrochozoans,
Annelids
|
Abstract
We
demonstrate the presence in leech hemolymph of high levels
of a peptide recognized by antiserum directed against bovine
chromacin. The purification of the chromacin-like peptide
was carried out by an acidic extraction, followed by solid
phase and high pressure gel permeation chromatography and
reversed-phase HPLC purification. Its sequence (GDFELPSIADPQATFESQRGPSAQQVDK)
was established by a combination of techniques, including
automated Edman degradation, MALDI-TOF measurement and DOT
immunobinding assays with anti-chromogranin A. Mass spectrometry
measurement revealed a m/z 3177Da, revealing the fact that
the molecule is phosphorylated. ELISA titrations performed
at each step of the purification revealed a major increase
in the level of the peptide (ca. 125 nmol/µl of coelomic fluid)
15 min after LPS exposure. The increase in chromacin-like
peptide levels is both time and concentration dependent. The
level of this peptide decreased significantly 4 hours after
LPS exposure. This report is the first discovery of a chromogranin
derived like peptide in invertebrates.
|
|
__________________________________________________________
Copyright © Neuroendocrinology Letters 2003
Society of Integrated Sciences
All rights reserved. No part may be reproduced, stored in a
retrieval system, or transmitted in any form or by any means,
electronic, mechanical, photocopying, recording, or ortherwise,
without prior written permission from the Editor-in-Chief.
The
latest statistics from the www.nel.edu
|
|
|
