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NEUROENDOCRINOLOGY
LETTERS
including
Psychoneuroimmunology, Neuropsychopharmacology,
Reproductive Medicine, Chronobiology
and Human Ethology, ISSN 0172–780X
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NEL
Vol.24 Nos.3/4, Jun-Aug 2003
ORIGINAL ARTICLE
Running
Title:
Testosterone and gonadotropin levels in men with dementia
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2003;
24:209–214
pii: NEL243403A10
PMID: 14523359
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The effects of photoperiod
on testes in rat:
A morphometric and immunohistochemical study
Ilter
Kus 1, Nusret Akpolat 2, Hakan Oner 3,
Ahmet Ayar 4,
Hýdýr Pekmez 1, Oguz Aslan Ozen
5 & Mustafa Sarsilmaz 1
1.
Department of Anatomy, Faculty of Medicine, Firat University,
Elazig;
2. Department of Pathology, Faculty of Medicine, Firat University,
Elazig;
3. Department of Histology & Embryology, Faculty of Veterinary,
Antalya University, Burdur;
4. Department of Pharmacology, Faculty of Medicine, Firat University,
Elazig;
5. Department of Anatomy, Faculty of Medicine, Zonguldak Karaelmas
University, Zonguldak;
TURKEY.
Submitted:
September 19, 2002
Accepted: October 22, 2002
Key
words:
photoperiod, testes, immunohistochemistry, rat
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Abstract
OBJECTIVE:
The aim of this study was to examine the effects of photoperiod
on testes in rat.
DESIGN:
For this purpose 21 male Wistar rats were used. Animals were
divided into three groups. Control rats in group I were kept
under 12 hrs light: 12 hrs dark conditions (12L: 12D) for
10 weeks. Animals in group II were exposed to long photoperiods
(18L: 6D), while rats in group III were exposed to short photoperiods
(6L: 18D) for 10 weeks. At the end of the experiment, all
animals were killed by decapitation and blood samples were
obtained. Serum testosterone levels were determined with the
use of a chemiluminescent enzyme immunoassay. The testes of
all rats were removed and weighed. Testicular tissue was processed
semiquantitative evaluation of immunohistochemical testosterone
staining. Intensity of immunostaining was determined on a
scale between 0 (no staining) and 5 (heavy staining). For
morphometric comparison, diameters of seminiferous tubules
in each group were measured.
RESULTS:
In rats exposed to long photoperiods, testicular weights,
diameters of seminiferous tubules and serum testosterone levels
were significantly increased as compared to those in control
rats. Whereas, exposure of rats to short photoperiods resulted
in significantly decrease of testicular weights, diameters
of seminiferous tubules and serum testosterone levels as compared
to those in control rats and rats maintained in long photoperiods.
Immunostaining of testosterone was moderate (3+) in control
rats, heavy (5+) in rats exposed to long photoperiods and
minimal (1+) in rats exposed to short photoperiods.
CONCLUSIONS:
The results of our study indicate that testicular functions
increase after exposure to long photoperiods and decrease
after exposure to short photoperiods.
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__________________________________________________________
Copyright © Neuroendocrinology Letters 2003
Society of Integrated Sciences
All rights reserved. No part may be reproduced, stored in a
retrieval system, or transmitted in any form or by any means,
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without prior written permission from the Editor-in-Chief.
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