Articles by author - Romuald Hudecek

Preparation and application of anti-peptide antibodies for detection of orphan cytochromes P450.

Hodek P, Hrdinova J, Macova I, Soucek P, Mrizova I, Burdova K, Kizek R, Hudecek J, Stiborova M.

Journal Article 2015; 36(Suppl 1): 38-45 PubMed PMID: 26757124  Keywords:  Animals, Antibodies:immunology, Antibody Formation, Blotting, Western, Cell Line, Tumor, Chickens, Cytochrome P-450 Enzyme System:immunology, Cytochrome P450 Family 2, Eggs, Electrophoresis, Gel, Two-Dimensional, Enzyme-Linked Immunosorbent Assay, Humans,.    Citation

OBJECTIVES: Cytochromes P450 (CYP) are monooxygenases, which metabolize mostly hydrophobic endogenous and exogenous compounds. CYPs withou.....

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Effect of dihydromyricetin on benzo[a]pyrene activation in rats.

Hodek P, Fousova P, Brabencova E, Moserova M, Pavek P, Anzenbacherova E, Brotanek J, Hudecek J, Frei E, Stiborova M.

Journal Article 2014; 35(Suppl 2): 158-168 PubMed PMID: 25638381  Keywords:  Animals, Benzo(a)pyrene:administration & dosage, Carcinogens:administration & dosage, DNA Adducts:administration & dosage, Flavonols:administration & dosage, Male, Rats, Rats, Wistar,.    Citation

OBJECTIVES: Flavanol dihydromyricetin (DHM) has been shown to counteract acute ethanol (EtOH) intoxication and reduce excessive E.....

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Quantification of interactions between cytochrome P450 2B4 and cytochrome b5 in a functional membrane complex.

Ječmen T, Ptáčková R, Kavan D, Cerná V, Hodek P, Stiborová M, Hudeček J, Sulc M.

Journal Article 2014; 35(Suppl 2): 114-122 PubMed PMID: 25638375  Keywords:  Animals, Aryl Hydrocarbon Hydroxylases:metabolism, Cell Membrane:metabolism, Cytochrome P450 Family 2, Cytochromes b5:metabolism,.    Citation

OBJECTIVES: The mammalian mixed function oxidase (MFO) system participates in hydroxylation of many hydrophobic endogenous compou.....

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Mapping of interaction between cytochrome P450 2B4 and cytochrome b5: the first evidence of two mutual orientations.

Sulc M, Jecmen T, Snajdrova R, Novak P, Martinek V, Hodek P, Stiborova M, Hudecek J.

Journal Article 2012; 33(Suppl 3): 41-47 PubMed PMID: 23353842  Keywords:  Animals, Aryl Hydrocarbon Hydroxylases:chemistry, Carbodiimides:chemistry, Chromatography, Liquid:methods, Cross-Linking Reagents:chemistry, Cytochrome P450 Family 2, Cytochromes b5:chemistry, Dimerization, Electrons, Mass Spectrometry:methods, Microsomes.    Citation

OBJECTIVES: The cytochrome P450 (P450) and cytochrome b5 are membrane hemoproteins composing together with flavoprotein NADPH:P450 reducta.....

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Comparison of activation of aristolochic acid I and II with NADPH:quinone oxidoreductase, sulphotransferases and N-acetyltranferases.

Martinek V, Kubickova B, Arlt V, Frei E, Schmeiser H, Hudecek J, Stiborova M.

Comparative Study 2011; 32(Suppl 1): 57-70 PubMed PMID: 22167209  Keywords:  Acetyltransferases:chemistry, Animals, Aristolochic Acids:chemistry, Biotransformation:physiology, Catalytic Domain, Cells, Cultured, DNA Adducts:metabolism, Enzyme Activation, Humans, Lactams:metabolism, Models, Molecular, Molecular Conformation, NAD(P)H.    Citation

OBJECTIVES: Ingestion of aristolochic acid (AA) is associated with development of urothelial tumors linked with aristolochic acid nephropa.....

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A one-electron oxidation of carcinogenic nonaminoazo dye Sudan I by horseradish peroxidase.

Semanska M, Dracinsky M, Martinek V, Hudecek J, Hodek P, Frei E, Stiborova M.

Journal Article 2008; 29(5): 712-716 PubMed PMID: 18987613  Keywords:  Carcinogens:chemistry, Chromatography, High Pressure Liquid, Chromatography, Thin Layer, Electrons, Horseradish Peroxidase:chemistry, Hydrogen Peroxide:chemistry, Magnetic Resonance Spectroscopy, Mass Spectrometry, Naphthols:chemistry, Oxidation-Reduction.    Citation

OBJECTIVES: The aim of the study was to examine oxidation of carcinogenic Sudan I by peroxidase and characterize the structure of its two .....

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