BACKGROUND: Nasal polyps (NPs) are one of the most common inflammatory mass lesions of the nose, affecting up to 0.5-4% of the population. The pathogenesis of NPs has been studied widely, but it is not clearly understood. A possible role of S. aureus in nasal polyposis has been suggested by numerous studies. This study aimed to map S.aureus colonisation in NP patients in the nose in comparison to healthy controls. MATERIAL AND METHODS: We identified Staphylococcus aureus in nasal mucosal swab, collected from 58 patients with nasal polyposis from the out-patient ENT clinic of the Faculty Hospital in Nitra. We compared them to 50 patients without symptoms of nasal obstruction or NP. Isolated bacterial strains were then further identified. RESULTS: In nasal mucosa membrane, results were not statistically significant. The selected population consisted of 108 patients, of which 58 (54%) had nasal polyps and 50 (46%) didnt. We collected the following information about patients from both groups: age, gender, smoker, presence of asthma, allergy and presence of Staphylococcus aureus by cultivation from nasal mucosa. In addition, for patients with nasal polyposis we have following variables, such as: presence inflammatory diseases, allergy to acylpyrine, cystic fibrosis. Out of 58 patients with nasal polyposis 15% (n=9) were found to have S.aureus in nasal mucosa membrane, compared to the healthy controls where 6% (n=3) of patients had S. aureus. CONCLUSION: Our results did not show that S. aureus found in nasal mucosa membrane is significantly different in patients with or without NP. However, association of the presence of S. aureus in patients with nasal polyposis with asthma, allergy and inflammation has been shown.