Thioredoxin is a potential therapy for light-induced photoreceptor degeneration in diabetic mice.

  Vol. 39 (8) 2019 Neuro endocrinology letters Journal Article   2019; 39(8): 561-566 PubMed PMID:  30927761    Citation

BACKGROUND: Retina degeneration is a set of disease that can be characterized by progressive loss of rod or cone photoreceptors that can experience higher light exposure compared with other parts of our body. MATERIAL AND METHODS: To investigate in diabetic mice the effects of different light on retinal photoreceptor and its related mechanisms, 4-6 weeks male Balb/c mices were randomly divided into control group, light treatment group (light 1 and light 2), light treatment with filter membrane group (LFM1 and LFM2), light treatment filter blue light group (LFB), diabetic group (DM), diabetic with light treatment group (DM+Light), diabetic light treatment with filter membrane group (DM+LFM1 and DM+LFM2), diabetic light treatment with filter blue light group (DM+LFB). Electroretinography (ERG) was used to assess retinal function. H&E staining was used to observe the morphology and measure the outer nuclear layer thickness in the retina. The related gene expression was detected by Real-time PCR. RESULTS: Light can significantly reduce the retinal function, outer nuclear layer thickness, and Trx expression. After different kinds of membranes and blue light filter treatments, the outer nuclear layer thickness, Nrf2, Trx expression can be improved in diabetic mice. However, the expression of Txnip and ASK1 was decreased. CONCLUSTION: Light can increase the photoreceptor damage in diabetic mice. The reduction of light intensity and change in the visible light components can reduce the light-induced damage in diabetic mice, and the mechanism may be related to up-regulation the antioxidant protein Trx expression.