Acute toxicity of magnetic nanoparticles in mice.

Gajdosíková A, Gajdosík A, Koneracká M, Závisová V, Stvrtina S, Krchnárová V, Kopcanský P, Tomasovicová N, Stolc S, Timko M.
  Vol. 27 (Suppl 2) 2006 Neuro endocrinology letters Comparative Study   2006; 27(Suppl 2): 96-99 PubMed PMID:  17159789    Citation  Keywords:  Animals, Drug Compounding, Female, Magnetics, Metal Nanoparticles:analysis, Mice, Mice, Inbred ICR, Polyesters:chemistry, Toxicity Tests,.   

OBJECTIVES: The acute toxicity of magnetic nanoparticles was effectively lowered by their encapsulation with poly(D,L lactide). In relation to the idea to use magnetic nanoparticles in development of new delivery systems suitable for targeted drug administration, the toxicological profile of five types of magnetic fluids was assessed in mice.

METHODS: The nanoprecipitation method was used to prepare magnetic fluids containing nanoparticles of Fe(3)O(4) encapsulated with biodegradable substances. The acute toxicity testing was performed according to OECD Test Guideline 425. In the pilot distribution study a special staining method was examined for the detection of Fe ions in body tissues of mice after intravenous administration of magnetic fluids.

RESULTS: The p.o. LD(50) values were greater than 2,000.0 mg/kg of body weight and i.v. LD(50) values were in the range of 231.7-558.9 mg/kg of body weight.

CONCLUSIONS: Of the magnetic nanoparticles tested, those encapsulated with poly(D,L lactide) were the most prospective for further in vivo testing.