The
insulin, glucose and cholesterol level and activity of lysosomal
enzymes in the course of the model alloxan diabetes
by Bozena Witek, Teodora Król, Adam Koataj, Ewa
Ochwanowska, Iwona Stanisawska & Alina Slewa
Keywords:
Alloxan diabetes, insulin level, glycaemia
level, cholesterol level, lysosomal enzymes, rabbits
Submitted:
July 23, 2001
Accepted: July 30, 2001
Abstract:
OBJECTIVES: The study was carried out on fifty male rabbits
of the New Zealand White breed. Diabetes was caused by a single,
intravenous alloxan injection. Rabbits which had glycaemia
7th day after the alloxan administration higher than 11 millimol/litre
were selected for the studies. They were divided into 5 groups:
I ñ control (without diabetes); II ñ 3-week diabetes; III
ñ 6-week diabetes; IV ñ 3-month diabetes; V ñ 6-month diabetes.
METHODS:
In control and experimental rabbits the activity of b-glucuronidase,
N-acetyl-b-glucosaminidase, lysosomal
acid phosphatase, leucine aminopeptidase, cathepsin D, and
lysosomal arylesterase was determined in lysosomal fractions
of the liver and kidney.
RESULTS:
Alloxan caused lowering of the activity of all the investigated
enzymes in the kidney and liver except lysosomal arylesterase.
CONCLUSION:
Alloxan injection caused a significant increase in the activity
of all the investigated enzymes. The advisable lysosomal enzymes
may be useful for the monitoring of the course and effectiveness
of diabetes therapy.
