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NEUROENDOCRINOLOGY
LETTERS
including
Psychoneuroimmunology, Neuropsychopharmacology,
Reproductive Medicine, Chronobiology
and Human Ethology, ISSN 0172–780X
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NEL
Vol.24 Nos.3/4, Jun-Aug 2003
ORIGINAL ARTICLE
Running
Title:
Ultrastructure of pinealocytes in mice implanted with Colon
38 adenocarcinoma
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2003;
24:269–273
pii: NEL243403A20
PMID: 14523369
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Ultrastructure
of pinealocytes in mice implanted with Colon 38 adenocarcinoma
Michal
Karasek 1, Anna Zieliñska 1, Krystyna
Marek 1, Jacek Swietoslawski 1 & Katarzyna
Winczyk 2
1.
Department of Electron Microscopy, Chair of Pathomorphology,
Medical University of Lodz;
2. Department of Experimental Endocrinology and Hormone Diagnostics,
Institute of Endocrinology; Medical University of Lodz, POLAND.
Submitted:
January 14, 2003
Accepted: February 3, 2003
Key
words:
pineal gland, pinealocyte, ultrastructure, colon cancer
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Abstract
OBJECTIVES:
Relationship between the pineal gland and neoplastic disease
has been repeatedly shown in many both experimental and clinical
studies. However, morphological studies of the pineal gland
in animals with experimentally-induced tumors are rare. Therefore,
we decided to investigate the ultrastructure of pinealocytes
in mice with implanted Colon 38 adenocarcinoma.
MATERIAL
AND METHODS: Male adult B6D2F1 mice were used in this
study. The animals were divided into two groups. Eight mice
were subcutaneously implanted with Colon 38 cell suspension,
whereas the other eight intact animals served as controls.
Three weeks after tumor implantation four animals from each
group were sacrificed by spinal cord dislocation at 12:00
h or 24:00 h. The pineal glands were removed and processed
for electron microscopic studies. The cross-sectional areas
of the pinealocyte and its nucleus, and relative volume of
mitochondria, Golgi apparatus, lysosomes, granular endoplasmic
reticulum, and lipid droplets, as well as the number of dense-core
vesicles were estimated using a digital analyzer connected
on-line to IBM-PC computer. Statistical analysis of the data
was performed using Student’s t-test and Snedecor F test.
RESULTS:
In the pineal glands of the tumor-bearing animals killed during
the daytime diminished size of pinealocytes and their nuclei,
decreased relative volume of granular endoplasmic reticulum,
lysosomes, and lipid droplets as well as decreased number
of dense-core vesicles were observed. On the contrary, the
relative volumes of mitochondria and Golgi apparatus were
increased in these animals. In the tumor-bearing animals killed
at night, however, increased cross-sectional areas of pinealocytes,
and decreased number of dense-core vesicles were observed.
CONCLUSIONS:
The results of our study suggest that the presence of the
malignant tumor influences the morphology of pineal cells
in mice. Considering the fact that different patterns of ultrastructural
changes were demonstrated in pinealocytes in different tumor
types in various species, it seems that the character of the
ultrastructural changes observed in tumor-bearing animals
depends on the animal species and tumor type.
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__________________________________________________________
Copyright © Neuroendocrinology Letters 2003
Society of Integrated Sciences
All rights reserved. No part may be reproduced, stored in a
retrieval system, or transmitted in any form or by any means,
electronic, mechanical, photocopying, recording, or ortherwise,
without prior written permission from the Editor-in-Chief.
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