Reproductive Medicine, Chronobiology
and Human Ethology, ISSN 0172780X
Vol.24 Nos.3/4, Jun-Aug 2003
Progesterone and Cytokines Production
effect of estradiol, but not progesterone, on the production
of cytokines in stimulated whole blood, is concentration-dependent
of Pharmacy and Medical Technology, University of Petra, Amman,
March 3, 2003
Accepted: March 7, 2003
estradiol, progesterone, TNF-a,
IFN-g, IL-10, IL-12
The purpose of this study was to determine the effects of
estradiol and progesterone on interferon-g (IFN-g),
interleukin (IL)-12, IL-10 and tumor necrosis factor-a (TNF-a)
productions in polyclonal activators (phytohemagglutinin+lipopolysaccharide)-stimulated
whole blood cultures.
Nineteen healthy males and females volunteered in the study.
Blood samples were drawn, diluted, and cultured for 24h with
different concentrations of estradiol, progesterone or hydrocortisone
and then PHA+LPS was added for another 24 h. The supernatant,
then, was harvested and assayed for IL-12 p70, IFN-g,
IL-10 and TNF-a.
At preovulatory concentrations, estradiol enhanced significantly
IFN-g, IL-12 and IL-10, but not
TNF-a, production levels and reversed
the suppressive effect of hydrocortisone in PHA+LPS stimulated
whole blood. While IL-10 levels kept increasing at pregnancy
estradiol concentrations, IFN-g,
IL-12 levels and IFN-g/IL-10 ratio
decreased significantly. No effect of progesterone on IL-12
p70, IFN-g, IL-10 and TNF production
levels was observed.
The present study shows that those pregnancy estradiol concentrations
(and higher) enhance the production of IL-10 and reduce IL-12,
IFN-g levels and IFN-g/IL-10
ratio in stimulated whole blood cells. Because of the known
IL-10 inhibitory actions on T helper (Th) 1 cells and monocytes/macrophages,
these high IL-10 levels keep Th2 cytokines favored during
pregnancy and may be useful in shifting Th1-mediated autoimmune
diseases towards non-pathogenic Th2 pathway.
Copyright © Neuroendocrinology Letters 2003
Society of Integrated Sciences
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