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NEUROENDOCRINOLOGY
LETTERS
including
Psychoneuroimmunology, Neuropsychopharmacology,
Reproductive Medicine, Chronobiology
and Human Ethology, ISSN 0172–780X
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NEL
Vol.24 Nos.3/4, Jun-Aug 2003
ORIGINAL ARTICLE
Running Title:
Melatonin and ischemia-reperfusion of testis
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2003;
24:170–172
pii: NEL243403A03
PMID: 14523352
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The
effect of prophylactic melatonin administration on reperfusion
damage in experimental testis ischemia-reperfusion
Ahmet
Ozturk 1, Abdulkerim Kasim Baltaci 2, Rasim
Mogulkoc 2 & Bahadir Ozturk 3
1.
Selcuk University, School of Medicine, Urology Department,
Konya, TURKEY.
2. Selcuk University, School of Medicine, Physiology Department,
Konya, TURKEY.
3. Selcuk University, School of Medicine, Biochemistry Department,
Konya, TURKEY.
Submitted:
March 7, 2003
Accepted: April 2, 2003
Key
words:
testis torsion, ischemia-reperfusion, malondialdehyde
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Abstract
AIM:
Torsion of testis, which is a urologic emergency case, is
generally treated by surgical detorsion procedure. However,
the resulting reperfusion and both ipsilateral and contralateral
testis damage caused thereby are important problems. This
study aims at investigating the administration of prophylactic
melatonin in order to reduce free radical damage that is caused
due to reperfusion after experimental testis torsion-detorsion
procedure.
MATERIAL
AND METHOD: The rats used in the study were allocated
to four groups, each containing 10 rats. Rats in Group I had
six hours of torsion (ischemia) followed by orchiectomy. Rats
in Group II had six hours of torsion, then received melatonin
(10 mg/kg/IM) and after that had detorsion (reperfusion).
Rats in Group III had detorsion after six hours of torsion
and serum physiologic administration (the same volume as melatonin).
Torsion and/or detorsion procedures were not applied in Group
IV (control). Ipsilateral, contralateral testis and plasma
Malondialdehyde (MDA) levels were determined in all groups.
RESULTS:
Tissue and plasma MDA levels in the group which had detorsion
were found to be significantly higher than those in the group
that had orchiectomy only (p<0.02). Ipsilateral and contralateral
testis MDA levels were identified to be significantly lower
in the group receiving prophylactic melatonin in comparison
to the group receiving serum physiologic (p<0.02). Plasma
and contralateral MDA levels correlated positively with MDA
levels identified in ipsilateral testis in all groups (rs=+0.89,
n=40, p=0.000).
CONCLUSION:
In cases where testis torsion is identified, administration
of melatonin just before torsion may reduce local and systemic
free radical damage.
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__________________________________________________________
Copyright © Neuroendocrinology Letters 2003
Society of Integrated Sciences
All rights reserved. No part may be reproduced, stored in a
retrieval system, or transmitted in any form or by any means,
electronic, mechanical, photocopying, recording, or ortherwise,
without prior written permission from the Editor-in-Chief.
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