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NEUROENDOCRINOLOGY LETTERS
including Psychoneuroimmunology, Neuropsychopharmacology,
Reproductive Medicine, Chronobiology
and Human Ethology, ISSN 0172–780X

NEL Vol.24 Nos.3/4, Jun-Aug 2003

ORIGINAL ARTICLE

The presence of 17-b estradiol in Mytilus edulis gonadal tissues:
Evidence for estradiol isoforms

2003; 24:137140
pii: NEL243403A02
PMID: 14523346

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Related PRESS RELEASE
Related Articles in this issue:
1. Preface - Guest Editorial
by Stefano
2. Stefano et al
| 3. Zhu et al (below)| 4. Cho et al - Review

 

The presence of 17-b estradiol in Mytilus edulis gonadal tissues:
Evidence for estradiol isoforms

Wei Zhu, Kirk Mantione, Dolisha Jones, Elliott Salamon, John J. Cho, Patrick Cadet & George B. Stefano

Neuroscience Research Institute, State University of New York College at Old Westbury, Old Westbury, New York, USA;

Submitted: July 22, 2003
Accepted: July 31, 2003

Key words:
estrogen, gonads, invertebrate, reproduction

 

Abstract

OBJECTIVES: In earlier studies, we demonstrate that 17-b -estradiol and an estrogen cell surface receptor can be found on various human cells, i.e., vascular endothelial, monocytes, and granulocytes, where they are coupled to nitric oxide release. We further demonstrated this phenomenon in the marine mussel Mytilus edulis ganglionic tissues. In the present report we sought to determine if estrogen can be found in M. edulis reproductive tissues.

MATERIAL & METHODS: We determined the presence of 17-b -estradiol via high pressure liquid chromatography (HPLC) and radioimmunoassay (RIA) in the animals gonads. This substance was further identified via nanoelectro-spray ionization quadrupole time of flight mass spectrometry (Q-TOF-MS).

RESULTS: 17-b -estradiol was identified and quantified in Mytilus gonads. Interestingly, we also determined that estradiol isoforms also were present in this tissue.

CONCLUSION: These data demonstrate that 17-b-estradiol and an estradiol isoform is present in M. edulis gonadal tissues, suggesting that they have functions related to reproduction. This further suggests that estrogen’s association with reproductive activities has a long evolutionary history and that this association began in invertebrates.

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