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NEUROENDOCRINOLOGY
LETTERS
including
Psychoneuroimmunology, Neuropsychopharmacology,
Reproductive Medicine, Chronobiology
and Human Ethology, ISSN 0172–780X
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NEL
Vol.24 No.1/2, Feb-Apr 2003
ORIGINAL ARTICLE
Melatonin
receptor tissular localization
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2003;
24:33–38
pii: NEL241203A04
PMID: 12743529
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MT1
melatonin receptor mRNA tissular localization by PCR amplification
Vincent-Joseph
Poirel, Cathy Cailotto, Dominique Streicher,
Paul Pévet, Mireille Masson-Pévet
& François Gauer
Laboratoire
de Neurobiologie des Rythmes, CNRS-UMR 7518, Université
Louis Pasteur, Strasbourg, FRANCE.
Submitted:
January 9, 2003 Accepted: January 15, 2003
Key
words:
Melatonin, pineal gland, circadian system, biological
rhythms, suprachiasmatic nuclei, receptors, quantitative PCR
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Abstract
OBJECTIVES:
The pineal gland transduces photoperiodic informations to
the neuroendocrine axis through the nocturnally melatonin
secretion. This hormonal message plays a major role in the
biological rhythm regulation. By autoradiography, more than
130 melatonin putative targets have been reported in the central
nervous system (CNS) and in peripheral tissues. However, cross-species
consensus concern only a few of them like the suprachiasmatic
nuclei (SCN), the master circadian clock, and the pars tuberalis
of the pituitary. Recently, mt1 melatonin receptor cDNA have
been cloned in several mammals providing us with new tools
to investigate its tissular location at the gene level. In
the present study, we report a screening for mt1 mRNA by RT-PCR
amplification of numerous tissue mRNA.
METHOD:
mRNA were extracted from a large variety of rat tissues. To
semi-quantify the melatonin receptor mRNA expression level,
each cDNA was amplified concomitantly with both b-actin
and mt1 specific primers.
RESULTS:
In central and peripheral tissues previously reported to bind
melatonin, strong PCR signals were logically observed. More
surprisingly, a vast majority of studied tissues express MT1
mRNA and then might be responsive to melatonin.
CONCLUSION:
Numerous biological functions express diurnal rhythmicity
and internal-synchronization. As, most of them apparently
do not receive any out-coming neuronal message from the SCN,
endocrine communication was proposed to support biological
rhythm synchronization. Our present data strengthen the idea
that the nocturnally restricted melatonin secretion could
be one internal zeitgeber that putatively distributes the
endogenous circadian rhythmicity to all tissues expressing
melatonin receptors.
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__________________________________________________________
Copyright © Neuroendocrinology Letters 2003
Society of Integrated Sciences
All rights reserved. No part may be reproduced, stored in a
retrieval system, or transmitted in any form or by any means,
electronic, mechanical, photocopying, recording, or ortherwise,
without prior written permission from the Editor-in-Chief.
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