Bozena
Witek, Ewa Ochwanowska, Alina Slewa &
Adam Kolataj
1.
Department of Genetics, Institute of Biology, Swietokrzyska
Academy, POLAND.
2. Institute of Genetics and Animal Breeding, Polish Academy
of Sciences in Jastrzebiec, Wólka Kosowska near Warsaw,
POLAND.
Submitted:
February 9, 2002
Accepted: February 11, 2002
Key words:
hydrocortisone, lysosomes, cathepsin D and L, alanine aminopeptidase,
leucine aminopeptidase,
N-acetyl-b-glucosaminidase, lysosomal
lipases, homeostasis, mice
Key words:
Nitric
Oxide, Sexual Maturation, GnRH, gonadotrophins
Abstract
OBJECTIVES:
Changes in the activity of cathepsin D and L, alanine aminopeptidase,
leucine aminopeptidase, N-acetyl-b-glucosaminidase,
lysosomal arylesterase and lysosomal lipase in the liver and
kidney of unselected and selected mice, subjected to 7.5 mg/kg
b.w. of hydrocortisone injection for 4 and 8 days.
METHODS:
The homogenates of the liver and kidney were subjected to
differentiated centrifuging and determination of studied enzymes.
RESULTS:
Injection of hydrocortisone caused an increase in the activity
of all investigated lysosomal enzymes in the liver and kidney
of mice.
CONCLUSION:
The reactions of selected mice were stronger in comparison
with unselected ones. The highest increase in the activity
investigated enzymes was observed after 8 days of hydrocortisone
injection.
Introduction
Glucocorticosteroids,
and first of all hydrocortisone (cortisol), have influence
on the metabolism of proteins, lipids and carbohydrates, and
only in physiological concentrations plays an important role
in regulatory processes, such as for instance in maintaining
intracellular homeostasis [1-4]. The metabolic effects of
hydrocortisone action arises from the type of the target tissue.
For example, in muscles, adipose and lymphatic tissues it
reveals catabolic activity, but in liver it stimulates synthesis
and glycogen storage [5-7]. In physiological conditions adrenal
cortex secretes about 20 mg of hydrocortisone within twenty-four
hours, but during stressful reaction its secretion can increase
even 10-times [8].
Environmentally unfavourable effects on animals cause, among
much else, stress responses. One of the cell arrangements
which take part in those responses is the lysosomal compartment.
The physiological importance of the lysosomal structure has
been described by numerous authors, which agree that it is
the principal site of intracellular degradation processes
[9-15]. It also a terminal compartment for the intracellular
transportation of newly synthesised lysosomal enzymes [16].
In our studies we observed the activity of some lysosomal
enzymes of mice subjected to hydrocortisone injections as
model factors affecting homeostasis of an organism. The aim
of our experiment was to determine the influence of the time
of administration of exogenous hydrocortisone on the activity
some proteolytical enzymes, glycosidases and lipases in the
liver and kidney of mice selected on economic feed consumption
and unselected ones, whose parents were matched at random.
Material
and Methods.
... ...
Results
... ...
Discussion
... ...
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