NEL
VOL. 22 No. 3
Original Article Full
text pdf
(93kb)
2001;
22:181-185 pii:
NEL220301A02
Effect
of melatonin administration on activities of some lysosomal
enzymes in the mouse by Bozena Witek, Ewa Ochwanowska, Adam Kolataj, Alina
Slewa & Iwona Stanislawska
OBJECTIVES:
Changes in the activity of b-glucuronidase,
N-acetyl-b-glucosaminidase, cathepsin
D and L, alanine aminopeptidase and lysosomal acid lipase
in lysosomal fractions of the liver and kidneys of mice, which
were administered 20 mg/kg b.w. of exogenous melatonin (N-acetyl-5-methoxytryptamine)
for 7 and 14 days were investigated.
METHODS: The slices of the liver and kidney were homogenized
in 0. 1M phosphate buffer, pH 7.0. Homogenates were subjected
to differentiated centrifuging and determination of studied
enzymes.
RESULTS: Melatonin caused lowering of the activity of all
the investigated lysosomal enzymes in the liver and kidney.
CONCLUSION: Administration of melatonin was caused the lowering
of the activity of the investigated lysosomal enzymes in comparison
with values in control groups.
