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Effects
of growth hormone (GH) and growth hormone releasing hormone
(GHRH) on progesterone and estradiol release from cultured rat
granulosa cells
by Boguslawa Baranowska, Magdalena Chmielowska, Monika Borowiec,
Krzysztof Roguski & Elzbieta Wasilewska-Dziubinska
Keywords:
GH; GH-RH; estradiol; progesterone
Abstract:
OBJECTIVES: It has been reported that GHRH-GH-IGF-1 system plays
an important role in the regulation of ovarian follicular development
and maturation.
METHODS:
In order to evaluate the direct effects of growth hormone releasing
hormone (GHRH) and growth hormone (GH) on steroidogenesis, the
effects of GHRH and GH on progesterone and estradiol release
from cultured rat granulosa cells were examined. The progesterone
and estradiol in supernatants were measured with RIA methods.
RESULTS:
Our results demonstrated that the addition of GH to the culture
medium produced a marked stimulation of progesterone and estradiol.
The stimulating effects were observed after administration of
GH in all concentrations: 1, 10, 100 nM during 60 and 120 mins
of incubation. During 240 mins of incubation the minimal stimulation
of progesterone and estradiol was found. However, GHRH administered
in 1, 10 and 100 nM did not change progesterone and estradiol
release from cultured granulosa cells.
CONCLUSION:
Growth hormone (GH) but not GHRH has direct stimulating effects
on progesterone release from cultured rat granulosa cells.
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